Publications Cell Physics Division

One hundred fifty years ago glia I cells were discovered as a second, non-neuronal, cell type in the central nervous system. To ascribe a function to these new, enigmatic cells, it was suggested that they either glue the neurons together (the Greek word "gamma lambda i alpha" means "glue") or provide a robust scaffold for them ("support cells"). Although both speculations are still widely accepted, they would actually require quite different mechanical cell properties, and neither one has ever been confirmed experimentally. We investigated the biomechanics of CNS tissue and acutely isolated individual neurons and glial cells from mammalian brain (hippocampus) and retina. Scanning force microscopy, bulk rheology, and optically induced deformation were used to determine their viscoelastic characteristics. We found that (i) in all CNS cells the elastic behavior dominates over the viscous behavior, (it) in distinct cell compartments, such as soma and cell processes, the mechanical properties differ, most likely because of the unequal local distribution of cell organelles, (iii) in comparison to most other eukaryotic cells, both neurons and glial cells are very soft ("rubber elastic"), and (iv) intriguingly, glial cells are even softer than their neighboring neurons. Our results indicate that glial cells can neither serve as structural support cells (as they are too soft) nor as glue (because restoring forces are dominant) for neurons. Nevertheless, from a structural perspective they might act as soft, compliant embedding for neurons, protecting them in case of mechanical trauma, and also as a soft substrate required for neurite growth and facilitating neuronal plasticity.

Recent intense interest in nanoparticle materials and nanoparticle-based contrast enhancement agents for biophysical applications gives new relevance to Mie scattering theory in its original context of application. The Mie theory still provides the most exact treatment of scattering from single nanoparticles of the noble metals. When recast in terms of modern electrodynamic formalism, the theory provides a concise closed-form representation for the scattered fields and also serves as a vehicle to elaborate the formal electrodynamic technique. The behavior of the Debye truncation condition for the multipole expansion is illustrated with numerical examples, clearly showing the features of the transition between the Rayleigh, dipole and higher order multipole approximations for the scattered fields. The classical Mie theory is an approximation in that only the transverse field components are included in the calculation. Extensions to the classical theory which include the effects of longitudinal fields are discussed and illustrated numerically. The example of scattering from multilayer composite particles is used to examine the feasibility of engineering spectral features of the scattering cross-section to target the requirements of specific applications.

We describe a novel microfluldic perfusion system for high-resolution microscopes. Its modular design allows pre-coating of the coverslip surface with reagents, biomolecules, or cells. A poly(dimethylsiloxane) (PDMS) layer is cast in a special molding station, using masters made by photolithography and dry etching of silicon or by photoresist patterning on glass or silicon. This channel system can be reused while the coverslip is exchanged between experiments. As normal fluidic connectors are used, the link to external, computer-programmable syringe pumps is standardized and various fluidic channel networks can be used in the same setup. The system can house hydrogel microvalves and microelectrodes close to the imaging area to control the influx of reaction partners. We present a range of applications, including single-molecule analysis by fluorescence correlation spectroscopy (FCS), manipulation of single molecules for nanostructuring by hydrodynamic flow fields or the action of motor proteins, generation of concentration gradients, trapping and stretching of live cells using optical fibers precisely mounted in the PDMS layer, and the integration of microelectrodes for actuation and sensing.