Microarray technology uses the sequence dependent hybridization<br> (binding) affinity of surface-bound oligonucleotide strands for the<br> quantification of complex nucleic acid mixtures. In spite of its huge<br> potential in life science and medicine, microarray oligonucleotide<br> hybridization remains far from being understood. Taking advantage of<br> microarray combinatorial possibilities we show that, although surface<br> bound, the hybridization affinities of single-base mismatched<br> oligonucleotides can be derived from first principles using parameters<br> from bulk.
Contact
Research Group Jona Kayser
Max Planck Institute for the Science of Light Staudtstr. 2 91058 Erlangen, Germany
